Identification of EDTA-Soluble Polysaccharides from Pea Epicotyl Cell Walls and Their Interaction with Xyloglucan
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چکیده
منابع مشابه
Identification of EDTA-Soluble Polysaccharides from Pea Epicotyl Cell Walls and Their Interaction with Xyloglucan
Nascent pectin and glucuronoxylan were prepared from membrane-bound enzymes obtained from pea epicotyls. They had previously been shown to exhibit a proteinand pH-dependent pattern of binding to cell wall ghosts and to xyloglucan extracted from cell walls prepared from pea epicotyls; maximum binding required a pH of 3-4, and the presence of cell wall proteins, namely assemblins. To determine wh...
متن کاملXyloglucan galactosyl- and fucosyltransferase activities from pea epicotyl microsomes.
Microsomal membranes from growing tissue of pea (Pisum sativum L.) epicotyls were incubated with the substrate UDP-[14C]galactose (Gal) with or without tamarind seed xyloglucan (XG) as a potential galactosyl acceptor. Added tamarind seed XG enhanced incorporation of [14C]Gal into high-molecular-weight products (eluted from columns of Sepharose CL-6B in the void volume) that were trichloroacetic...
متن کاملSoluble Cell Wall Polysaccharides Released from Pea Stems by Centrifugation : I. EFFECT OF AUXIN.
The metabolism of polysaccharides by pea stem segments treated with and without auxin was investigated using a centrifugation technique for removing solution from the free space of the cell wall. Glucose is the predominant sugar in both the ethanol-soluble and ethanol-insoluble fractions of the cell wall solution extracted with water. In the water-soluble, ethanol-insoluble polysaccharides, ara...
متن کاملRole of xyloglucan in primary cell walls.
The plant cell wall is a highly complex and dynamic structure made up of cellulose, hemicelluloses, pectins, lignin, and numerous proteins and inorganic molecules (Carpita and McCann, 2000). The hemicellulose xyloglucan (XyG) is found in the cell walls of all vascular plants and is the major hemicellulose in the primary cell wall of eudicots and nongraminaceous monocots, where it is believed to...
متن کاملFractionation of xyloglucan fragments and their interaction with cellulose.
Tamarind seed xyloglucan was partially degraded with a purified endoglucanase (endoV) from Trichoderma viride. Analysis by high-performance anion-exchange chromatography showed that this digest was composed of fragments consisting of 1 to 10 repeating oligosaccharide units ([xg]1-[xg]10). To study the adsorption of xyloglucan fragments to cellulose in detail, this digest was fractionated on Bio...
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ژورنال
عنوان ژورنال: American Journal of Plant Sciences
سال: 2011
ISSN: 2158-2742,2158-2750
DOI: 10.4236/ajps.2011.22016